Jin-Hee Lee

Postdoctoral ResearcherJin-hee-Lee

Education

  • B.S., Chemistry, Hanyang University, Seoul
  • M.S., Organic Chemistry, Hanyang University, Seoul
  • Ph.D., Chemistry, University of Illinois at Urbana-Champaign

Research Description

Histone posttranslational modification is one of the most important epigenetic mechanisms by which expression or repression of specific gene activity is governed.  Jin-Hee Lee’s research focuses on characterization of histone-modifying enzymes, such as histone acetyltransferase NuA4, and demethylases Jmjd2 family.  Lee is particularly interested in understanding the function of reader domains in these enzyme complexes.  In order to achieve this goal, she uses recombinantly prepared “designer” nucleosomes with specific PTMs and probe effect of “reader” binding during enzyme-dependent histone modifications.   She also focuses on linking the activity of specific histone PTMs to a prostate cancer state.  For this study, she utilizes histone peptide microarray technology to screen for biologically significant histone PTMs in a hope to develop it into a diagnostic biomarker as well as a prognostic tool for metastatic prostate cancer.

Honors

NSF Travel Awards for UW System Postdoctoral Scholars, 2014

Postdoctoral Research Grant, DOD Prostate Cancer Research Program (PC094414), 2012-2013

Hanyang University, Seoul Korea, Scholarship for academic excellence, 2001-2003

Selected Publications

  • Lee JH, Thorson JS. Site-specific protein glycosylation by bacterial N-glycosyltransferase.  2014, Submitted
  • Su Z, Boersma MD, Lee JH, Oliver SS, Liu S, Garcia BA, Denu JM.  ChIP-less Analysis of Chromatin States.    Epigenetics & Chromatin2014, 7:7
  • Circello BT, Miller CG, Lee JH, van der Donk WA, Metcalf WW.  The antibiotic dehydrophos is converted to a toxic pyruvate analog by peptide bond cleavage in Salmonella enterica.   Antimicrob. Agents Chemother. 2011, 55, 3357
  • Lee JH*, Bae B*, Kuemin M, Circello BT, Metcalf WW, Nair SK, van der Donk WA.  Characterization and crystal structure of DhpI, a novel phosphonate O-methyltransferase involved in dehydrophos biosynthesis.   PNAS, 2010, 107, 17557 (*Equal contribution)
  • Circello BT, Eliot AC, Lee JH, van der Donk WA, Metcalf WW.  Molecular cloning and heterologous expression of the dehydrophos biosynthetic gene cluster.  Chem. Biol. 2010, 17, 402
  • Lee JH, Evans BS, Li G, Kelleher NL, van der Donk WA.  In vitro characterization of a heterologously expressed nonribosomal peptide synthetase involved in phosphinothricin tripeptide biosynthesis. Biochemistry, 2009, 48, 5054
  • Kim W, Lee JH, Kang J, Cho CG. Diels–Alder cycloadditions of 3-phenylamino-5-bromo-2-pyrone for the synthesis of constrained α-amino acid derivatives. Tetrahedron Lett. 2004, 45, 1683
  • Lee JH, Cho CG. Regioselective Pd-catalyzed aminations of 3-amino-5-bromo-2-pyrones. Tetrahedron Lett. 2003, 44, 65
  • Lee JH, Kim W, Lee YY, Cho CG. Stille couplings of 3-(trimethylstannyl)-5-bromo-2-pyrone for the syntheses of 3-aryl-5-bromo-2-pyrones and their ambient dienyl characters. Tetrahedron Lett. 2002, 43, 5779
  • Lee JH, Park JS, Cho CG. Regioselective synthesis of 3-alkynyl-5-bromo-2-pyrones via Pd-catalyzed couplings on 3,5-Dibromo-2-pyrone.  Organic Lett. 2002, 4, 1171